Morandim-Giannetti A.D.A.Rubio S.R.Nogueira R.F.Ortega F.D.S.Magalhaes Junior O.Schor P.Bersanetti P.A.2019-08-202019-08-202018GIANNETTI, Andreia de Araujo Morandim; RUBIO, SAMANTHA REGINA; NOGUEIRA, REGINA FREITAS; ORTEGA, FERNANDO DOS SANTOS; MAGALHÃES JUNIOR, OCTAVIANO; SCHOR, PAULO; BERSANETTI, PATRÍCIA ALESSANDRA. Characterization of PVA/glutaraldehyde hydrogels obtained using Central Composite Rotatable Design (CCRD). JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, v. 106B, p. 1558-1566, 2018.1552-4981https://repositorio.fei.edu.br/handle/FEI/1712© 2017 Wiley Periodicals, Inc. Hydrogels are made from natural or synthetic polymers and, currently, they have many biomedical applications. In this work, the conditions for obtaining a hydrogel with similar physicochemical characteristics to the vitreous humor were defined using polyvinyl alcohol (PVA) and glutaraldehyde (GLUT) as cross-linker. The concentration of PVA and GLUT were modified, and their effect was analyzed in terms of the refractive index, density, and dynamic viscosity. The hydrogel which was obtained using 3.98% (w/V) of PVA, 3.13 mL (1.57 g) of GLUT in 100 mL, and the initial pH of 7.2 showed similar characteristics to the vitreous humor (density = 1.0174 ± 0.0050 g mL −1 , dynamic viscosity = 3.7425 ± 0.1800 mPa s and refractive index = 1.3410 ± 0.0010). The hydrogels were further investigated by rheological measurements, infrared spectroscopy, differential scanning calorimetry, X-ray diffraction and determination of swelling degree. The reticulation with GLUT promoted an increase in viscosity and glass transition temperature. On the other hand, it stimulated a decrease in the swelling degree, crystallinity, melting temperature, and intensity of the band related to the -OH bond, compared with the PVA without reticulation. The reticulated hydrogel displayed Newtonian behavior and a higher apparent viscosity than the PVA. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1558–1566, 2018.Acesso RestritoArtigo10.1002/jbm.b.33958design of experimentsglutaraldehydehydrogelPVAvitreous humor