Creative Commons "Este é um artigo publicado em acesso aberto sob uma licença Creative commons (CC BY 4.0). Fonte: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85098602226&origin=inward. Acesso em: 18 jan. 2023.FERREIRA, C. L.JARDINI, M. A. N.NUNES, C. M. M.BERNARDO, D. V.CASARIN, R. C. V.GEDRAITE, E. DOS. S.MATHIAS, M. A.LIU, F.MENDONÇA, GMENDONÇA, B. D. S.SANTAMARIA, M. P.2022-01-122022-01-122021-02-05FERREIRA, C. L.; JARDINI, M. A. N. ; NUNES, C. M. M.; BERNARDO, D. V.; CASARIN, R. C. V. ; GEDRAITE, E. DOS. S. ; MATHIAS, M. A.; LIU, F.; MENDONÇA, G. ; MENDONÇA, B. D. S. ; SANTAMARIA, M. P. Electrical stimulation enhances early palatal wound healing in mice. Archives of Oral Biology, v. 122, 2021.1879-1506https://repositorio.fei.edu.br/handle/FEI/3587© 2020 Elsevier LtdBackground/objective: Electrical stimulation (ES) has been used to treat chronic wound and other clinical applications, showing favorable results in wound closure. It was hypothesized that ES can present a positive effect on oral mucosa healing. The aim of this study was to investigate the effects of ES during the palatal mucosa early healing process in Swiss mice. Methods: Ninety animals were divided into two groups: Control (C; n = 45), which received Sham ES applications, and Test (ES; n = 45), which received ES (100 μA; 9 kHz; 660 mVpp) once a day for 3 days. A full thickness wound was performed with a 1.5 mm diameter biopsy punch in the hard palate. Histologically, the following parameters were evaluated: palatal wound closure and epithelial and connective wound edge distance (EED and CED). Furthermore, IL-1β, IL-6, IL-10 TNF-α, and VEGF cytokine levels were evaluated by multiplex assay. The percentage of collagen fibers was assessed using the polarization method and the Smad proteins using the immunofluorescence method. Results: Palatal wound closure presented a significant reduction on day 5 in the ES group (p = 0.01). Additionally, both EED and CED were shorter for all time points in the ES group (p < 0.05), and the inflammatory markers IL-6, IL-10, TNF-α, and VEGF were reduced (p < 0.05). There were no differences in collagen fibers and phospho-Smad2 between the groups. Conclusion: ES had a positive effect on early palatal wound closure outcomes, as well as on inflammatory markers.Acesso AbertoArtigo10.1016/j.archoralbio.2020.105028Electrical stimulationGingival recessionHard palateOral mucosaRe-epithelializationWound healing