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URI permanente para esta coleçãohttps://repositorio.fei.edu.br/handle/FEI/5120
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Artigo de evento 2 Citação(ões) na Scopus MEK - Multivariables device development for enzymes kinetics experiment in microgravity(2012-10-01) LA NEVE, A.; LUCARINI, A. C.; BEILODI, M.; MELO, M. A. A.; PEREIRA, A. N.The purpose of this experiment was to realize enzymatic bioreactions in microgravity, on board the ISS, as part of the investigations that are being done by this group, both in soil and on board sounding rockets. Though microgravity effects on some enzymes have been investigated and analysed by some researchers, results are contradictory, and there is still a long way to go to reach a full understanding of their behaviour. The knowledge of the influence of microgravity on enzymatic catalysis may lead to a better comprehension of cellular metabolism principles, and consequently to possible implementations and applications of enzymatic biosensors in space. For this experiment it was necessary to develop and implement a specific device, MEK, to allow that the mixture of liquids and multiple enzymatic bioreactions might be realized correctly in space, and be later easily recovered for biochemical analysis on earth. Invertase and lipase enzymes were used in the experiment, mainly due to the importance and large use they have in food, pharmaceutical and fine chemicals industry processes. Each of them was tested with different reagent concentrations, for proper kinetic data collection, in a total of 15 samples. The implementation of MEK device presented some severe challenges, in order to satisfy the necessary requirements to fly on the Brazilian Centenary Mission aboard the International Space Station. MEK device consists of a mechanical part, which contains 15 reaction chambers, especially designed for this purpose, for the mixture of enzyme and substrate liquids, with temperature sensors, and electronic circuits, to ensure monitoring, data acquisition, correct control activation, timing, and heating of liquids up to the correct temperature, which is necessary to interrupt each biochemical reaction. Enzymes and correspondent substrates were placed in two compartments of each reaction chamber, and were mixed by the action of a piston. MEK device was implemented with protection system requirements, for unexpected temperature elevation and threefold sealing, among others, to ensure safety for the ship and the crew. The experiment was carried out in three series, as a whole, to test the kinetics of invertase, free lipase and immobilized lipase enzymes, at five different concentrations for each respective substrate. The results were satisfactory, both regarding the equipment functioning and the biochemical reactions. The experiment however needs to be repeated several times in order to confront data, which are to be collected in different situations, and also to ensure statistical repeatability of results. ©(2012) by the International Astronautieal Federation.Artigo de evento 0 Citação(ões) na Scopus Developing technological knowledge in microgravity missions by matching partners expertise(2013-07-09) LA NEVE, A.; CORREA JUNIOR, F. DE A.The article takes into account the cooperative work between Centro Universitário da FEI (FEI), Agênda Espacial Brasileira (AEB), Academia Brasileira de Ciências (ABC), Instituto National de Pesquisas Espaciais (INPE) and Instituto de Aerondutica e Espaço (IAE) in the AEB Brazilian microgravity missions. It brings about some of the initial cares that were taken in order to better define and establish, in cooperation with the researchers, the onboard experiments for the sounding rockets (VS-30 and VSB-30) and for the International Space Station (ISS) by the establishing of researchers' reasonable requirements, by the definition of the payload/experiment interfaces and by the specification of the ground facilities needed for the experiment support. Inside this context, some mechanical, electronic and technical aspects of FEI experiments were analyzed to eventually assure a payload ready for a successful mission. The experiments that were sent on different missions by FEI were based on two main research lines-biotechnology and materials - for the study of enzymatic reactions and carbon nanotubes. This required that special devices be developed to perform the experiment in microgravity automatically. At each new flight an upgraded version of the device was implemented, based on the previous experience The article exploits some aspects of the design, development, assembling, test from missions of different characteristics (suborbital and orbital), and comments on what was learned in the process. This would finally lead to a know-how acquisition that resulted in a steady improvement of the projects in successive missions with microgravity experiments.Artigo de evento 0 Citação(ões) na Scopus Aspects of risk of management in brazilian microgravity experiments: A case of study(2014-10-03) LA NEVE, A.; CORRÊA, F. A.Copyright © 2014 by the International Astronautical Federation. All rights reserved.Learning is a process strengthened by the praxis. The subject of the present article is to verify possible methodologies to mitigate risks in aerospace projects regarding such specific cases as new developments for future microgravity missions at Centro Universitário da FEI (FEI). The base of this study is the knowledge and experience acquired in projects in some microgravity missions in which FEI participated. FEI has been present right from the beginning of the Microgravity Program of the Brazilian Space Agency (AEB) in 2002 (CUMÃ Mission), developing microgravity experiments for national sounding rockets and the International Space Station (ISS). As a matter of fact, its involvement in the aerospace field is previous to the AEB Microgravity Program, and it started with the Space Shuttle STS-95 (Discovery mission, 1998), and soon after that with the sounding rocket missions São Marcos (1999) and Lençóis Maranhenses (2000). From mission to mission different scenarios appeared to the project management, popping up new barriers to overcome and bringing back old ones to the present: a set of occurrences in a comprehensive amount of uncertainties that face the manager skills to deal with. This paper intend to suggest some techniques to increase the success rate by implementing a risk management project with the evaluation of possible methodologies for risk mitigation projects, proposing recommendations related to the management of aerospace projects based primarily on theory, and then on known and successful practices adopted in projects already completed. A fundamental step for the risk comprehension is to identify the sources of uncertainties and deleterious consequences, or, in other words, the risk factors. The identification of common risk factors is investigated in bibliography, by surveys and by the use of a DELPHY methodology in order to validate the factors identified as significant risk sources and their root causes, including, in addition, the point of view of some other expert managers. This article is a particular case study of a broader survey that is being elaborated in the dissertation one of the authors', at the National Institute for Space Research, at São José dos Campos, Brazil. ia.