DSC evaluation of cross-link development induced by laccases in corneas

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dc.contributor.authorAndreia de Araújo Morandim Giannetti
dc.contributor.authorCARVALHO, T. S.
dc.contributor.authorDE ANDRADE NETO, J. C.
dc.contributor.authorBERSANETTI, P. A.
dc.contributor.authorOrcidhttps://orcid.org/0000-0002-5428-768X
dc.date.accessioned2022-12-01T06:03:18Z
dc.date.available2022-12-01T06:03:18Z
dc.date.issued2022-11-25
dc.description.abstract© 2022, Akadémiai Kiadó, Budapest, Hungary.Several studies have been developed aiming at new treatments for ophthalmological problems such as keratoconus, an eye disease that leads to the reduction of cross-links between the corneal collagen fibrils. Corroborating these studies, this work evaluated the increase of cross-linking in enucleated porcine eyes treated with catechin in the presence of laccase. The ideal conditions for the treatment of the cornea to promote the development of cross-links were determined through the application of a design of experiments. The input variables were the concentration of laccase and the reaction temperature, and the output variable was the enthalpy of corneal denaturation. The DSC results show that the ideal laccase concentration was 8.0 mg mL−1 and the temperature was 36.0 °C. The catechin concentration used was 5.0 mg mL−1. This condition led to a 10.8% increase in the denaturation temperature and a 110.3% increase in the denaturation enthalpy. Enzymatic degradation tests showed that within 24 h of digestion the group treated with laccase in the presence of catechin was more resistant compared to the control group. A decrease of 34.7 and 55.6% in the relative area and relative mass of corneas in the control group was verified, while for the group treated with laccase these values were 41.7 and 8.5%, respectively.
dc.identifier.citationGIANNETTI, A. A. M.; CARVALHO, T. S.; DE ANDRADE NETO, J. C.; BERSANETTI, P. A. DSC evaluation of cross-link development induced by laccases in corneas. Journal of Thermal Analysis and Calorimetry, Nov. 2022.
dc.identifier.doi10.1007/s10973-022-11783-w
dc.identifier.issn1588-2926
dc.identifier.urihttps://repositorio.fei.edu.br/handle/FEI/4647
dc.relation.ispartofJournal of Thermal Analysis and Calorimetry
dc.rightsAcesso Restrito
dc.subject.otherlanguageCornea
dc.subject.otherlanguageCross-linking
dc.subject.otherlanguageDSC
dc.subject.otherlanguageLaccase
dc.titleDSC evaluation of cross-link development induced by laccases in corneas
dc.typeArtigo
fei.scopus.citations0
fei.scopus.eid2-s2.0-85142512736
fei.scopus.subject% reductions
fei.scopus.subjectCollagen fibrils
fei.scopus.subjectCondition
fei.scopus.subjectControl groups
fei.scopus.subjectCornea
fei.scopus.subjectCross linking
fei.scopus.subjectDSC
fei.scopus.subjectEnucleated porcine eye
fei.scopus.subjectEye disease
fei.scopus.subjectLaccases
fei.scopus.updated2024-05-01
fei.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85142512736&origin=inward
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