Scaffolds’ production based on calcium aluminate blends and their biological properties

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dc.contributor.authorde Las Nieves Gonzalez Vicuna I.
dc.contributor.authorGrancianinov K.J.S.
dc.contributor.authordos Santos K.W.
dc.contributor.authordos Santos Ortega F.
dc.contributor.authorde Camargo Reis Mello D.
dc.contributor.authorde Vasconcellos L.M.R.
dc.contributor.authorCosta M.S.
dc.contributor.authorPandolfelli V.C.
dc.contributor.authorde Oliveira I.R.
dc.date.accessioned2019-08-20T00:17:41Z
dc.date.available2019-08-20T00:17:41Z
dc.date.issued2019
dc.description.abstract© 2019, Sociedade Brasileira de Engenharia Biomedica.Purpose: Scaffolds are artificial structures that provide a temporary template to withstand mechanical loads in vivo during the process of bone regeneration. They should present a highly porous three-dimensional structure with an interconnected network of pores for the tissue growth and the transport of nutrients and metabolic residues. Additionally, scaffolds must be easily manufactured in different shapes and sizes. Methods: A 4 wt% of additives (alumina, zirconia, zinc oxide, hydroxyapatite, or tricalcium phosphate) was added to CAC (calcium aluminate cement), and these blends were used for scaffolds preparation via polymeric sponge replica. Aqueous suspensions were prepared with different solid contents. Sponges impregnated with 55 wt% solid were evaluated for the decomposition temperature by thermogravimetric analysis. The scaffolds microstructures were characterized by scanning electron microscopy and mercury intrusion porosimetry. The relative density was also calculated considering the theoretical density. The scaffolds (60 wt%) were characterized by cytotoxicity assay, total protein content, alkaline phosphatase activity, mineralized bone-like nodule formation, and cell adhesion. Results: The blends prepared with 60 and 55 wt% solid were most suitable for sponge impregnation, resulting in scaffolds with higher fraction of open porosity. The tricalcium phosphate blends presented the best results regarding mineralized nodules. Conclusion: The MTT tests and osteoblast cells adhered to the scaffolds provided predictive evidence of biocompatibility of the material which, associated with the satisfactory results obtained in the cell differentiation tests (ALP), suggested that the scaffolds developed are promising materials. Additionally, all scaffolds allowed the mineralized matrix formation which is a key issue for osteogenesis.
dc.description.firstpage131
dc.description.issuenumber2
dc.description.lastpage141
dc.description.volume35
dc.identifier.citationDE LAS NIEVES GONZÁLEZ VICUNA, IRANEL; GRANCIANINOV, KAREN JULIE SANTOS; DOS SANTOS, KENNEDY WALLACE; DOS SANTOS ORTEGA, FERNANDO; DE CAMARGO REIS MELLO, DAPHNE; DE VASCONCELLOS, LUANA MAROTTA REIS; COSTA, MARICILIA SILVA; PANDOLFELLI, Victor Carlos; DE OLIVEIRA, IVONE REGINA. Scaffolds? production based on calcium aluminate blends and their biological properties. RESEARCH ON BIOMEDICAL ENGINEERING, v. 35, n. 2, p. 131-141, 2019.
dc.identifier.doi10.1007/s42600-019-00015-0
dc.identifier.issn2446-4740
dc.identifier.urihttps://repositorio.fei.edu.br/handle/FEI/2354
dc.relation.ispartofResearch on Biomedical Engineering
dc.rightsAcesso Restrito
dc.subject.otherlanguageCalcium aluminate blends
dc.subject.otherlanguageCell culture
dc.subject.otherlanguageScaffolds
dc.subject.otherlanguageSolid content
dc.subject.otherlanguageSponge replica
dc.titleScaffolds’ production based on calcium aluminate blends and their biological properties
dc.typeArtigo
fei.scopus.citations2
fei.scopus.eid2-s2.0-85068311490
fei.scopus.subjectAlkaline phosphatase activity
fei.scopus.subjectCalcium aluminate
fei.scopus.subjectCalcium aluminate cement
fei.scopus.subjectDecomposition temperature
fei.scopus.subjectMercury intrusion porosimetry
fei.scopus.subjectSolid contents
fei.scopus.subjectSponge replica
fei.scopus.subjectThree-dimensional structure
fei.scopus.updated2024-03-04
fei.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85068311490&origin=inward
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